Parts of Cowdenbeath and Lochgelly have been prioritised for urban tree planting.
Woodland Trust Scotland has identified the areas as part of its efforts to improve health and wellbeing through increased tree cover.
Anna Perks, Scotland’s tree equity lead at the Woodland Trust, said: "If we are to plant urban trees, we want to plant them where they will make the most difference.
"Let’s put more trees where people need them most."
The trust is encouraging schools and community groups in Fife to apply for its free tree packs, which are distributed twice a year.
Applications are now open for November delivery.
Cowdenbeath and Lochgelly are two of the areas which will be prioritised to maximise the scheme’s impact.
The trust uses the Tree Equity Score UK tool to identify communities most in need of trees.
The tool factors in canopy cover, health, deprivation, urban heat, and air quality.
Ms Perks said: "Trees lift our mood, clean our air, shade our streets, reduce stress, nurture our wildlife, and help create attractive, healthy places we enjoy spending time in.
"Urban trees are linked to better health and wellbeing."
Fife Climate Forest, which is also supported through the free trees scheme, works with communities to plant and care for trees across the region.
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